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i script cdna synthesis kit  (Bio-Rad)


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    Bio-Rad i script cdna synthesis kit
    p53 expression and activity in SH-SY5Y AS cellular model. ( A – D ) Cell lysates from SH-SY5Y (control and UBE3A − cells), were used for western blotting analysis using specific antibodies to total p53 ( A , B ) and phosphorylated p53 ( C , D ). A ‘stain-free protein normalization’ method was used for the normalization of bands to total protein in blots, eliminating the need for housekeeping proteins. Data are expressed as percentage versus control cells and are mean ± SEM of at least three independent experiments Comparisons between control and UB3A − were performed using an unpaired two-tailed Student’s t -test; * p < 0.05, ** p < 0.01, UBE3A − vs. control. ( E ) SHSY-5Y (control and UBE3A − ) cells were collected for RNA extraction and retro transcription to <t>cDNA.</t> A real-time PCR analysis was performed to monitor mRNA expression of p53-traget genes (i.e., Bax, p21, and MDM2). Data are expressed as fold change relative to Control cells (set to 1) and represent mean ± SEM of four independent experiments. Comparisons between Control and UBE3A − were performed using an unpaired two-tailed Student’s t -test; *** p < 0.001.
    I Script Cdna Synthesis Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 6234 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/i script cdna synthesis kit/product/Bio-Rad
    Average 99 stars, based on 6234 article reviews
    i script cdna synthesis kit - by Bioz Stars, 2026-03
    99/100 stars

    Images

    1) Product Images from "Dysfunction of the Autophagy System and MDM2–p53 Axis Leads to the Accumulation of Amyloidogenic Proteins in Angelman Syndrome Models"

    Article Title: Dysfunction of the Autophagy System and MDM2–p53 Axis Leads to the Accumulation of Amyloidogenic Proteins in Angelman Syndrome Models

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms262211032

    p53 expression and activity in SH-SY5Y AS cellular model. ( A – D ) Cell lysates from SH-SY5Y (control and UBE3A − cells), were used for western blotting analysis using specific antibodies to total p53 ( A , B ) and phosphorylated p53 ( C , D ). A ‘stain-free protein normalization’ method was used for the normalization of bands to total protein in blots, eliminating the need for housekeeping proteins. Data are expressed as percentage versus control cells and are mean ± SEM of at least three independent experiments Comparisons between control and UB3A − were performed using an unpaired two-tailed Student’s t -test; * p < 0.05, ** p < 0.01, UBE3A − vs. control. ( E ) SHSY-5Y (control and UBE3A − ) cells were collected for RNA extraction and retro transcription to cDNA. A real-time PCR analysis was performed to monitor mRNA expression of p53-traget genes (i.e., Bax, p21, and MDM2). Data are expressed as fold change relative to Control cells (set to 1) and represent mean ± SEM of four independent experiments. Comparisons between Control and UBE3A − were performed using an unpaired two-tailed Student’s t -test; *** p < 0.001.
    Figure Legend Snippet: p53 expression and activity in SH-SY5Y AS cellular model. ( A – D ) Cell lysates from SH-SY5Y (control and UBE3A − cells), were used for western blotting analysis using specific antibodies to total p53 ( A , B ) and phosphorylated p53 ( C , D ). A ‘stain-free protein normalization’ method was used for the normalization of bands to total protein in blots, eliminating the need for housekeeping proteins. Data are expressed as percentage versus control cells and are mean ± SEM of at least three independent experiments Comparisons between control and UB3A − were performed using an unpaired two-tailed Student’s t -test; * p < 0.05, ** p < 0.01, UBE3A − vs. control. ( E ) SHSY-5Y (control and UBE3A − ) cells were collected for RNA extraction and retro transcription to cDNA. A real-time PCR analysis was performed to monitor mRNA expression of p53-traget genes (i.e., Bax, p21, and MDM2). Data are expressed as fold change relative to Control cells (set to 1) and represent mean ± SEM of four independent experiments. Comparisons between Control and UBE3A − were performed using an unpaired two-tailed Student’s t -test; *** p < 0.001.

    Techniques Used: Expressing, Activity Assay, Control, Western Blot, Staining, Two Tailed Test, RNA Extraction, Real-time Polymerase Chain Reaction



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    p53 expression and activity in SH-SY5Y AS cellular model. ( A – D ) Cell lysates from SH-SY5Y (control and UBE3A − cells), were used for western blotting analysis using specific antibodies to total p53 ( A , B ) and phosphorylated p53 ( C , D ). A ‘stain-free protein normalization’ method was used for the normalization of bands to total protein in blots, eliminating the need for housekeeping proteins. Data are expressed as percentage versus control cells and are mean ± SEM of at least three independent experiments Comparisons between control and UB3A − were performed using an unpaired two-tailed Student’s t -test; * p < 0.05, ** p < 0.01, UBE3A − vs. control. ( E ) SHSY-5Y (control and UBE3A − ) cells were collected for RNA extraction and retro transcription to <t>cDNA.</t> A real-time PCR analysis was performed to monitor mRNA expression of p53-traget genes (i.e., Bax, p21, and MDM2). Data are expressed as fold change relative to Control cells (set to 1) and represent mean ± SEM of four independent experiments. Comparisons between Control and UBE3A − were performed using an unpaired two-tailed Student’s t -test; *** p < 0.001.
    I Script Cdna Synthesis Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/i script cdna synthesis kit/product/Bio-Rad
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    Image Search Results


    p53 expression and activity in SH-SY5Y AS cellular model. ( A – D ) Cell lysates from SH-SY5Y (control and UBE3A − cells), were used for western blotting analysis using specific antibodies to total p53 ( A , B ) and phosphorylated p53 ( C , D ). A ‘stain-free protein normalization’ method was used for the normalization of bands to total protein in blots, eliminating the need for housekeeping proteins. Data are expressed as percentage versus control cells and are mean ± SEM of at least three independent experiments Comparisons between control and UB3A − were performed using an unpaired two-tailed Student’s t -test; * p < 0.05, ** p < 0.01, UBE3A − vs. control. ( E ) SHSY-5Y (control and UBE3A − ) cells were collected for RNA extraction and retro transcription to cDNA. A real-time PCR analysis was performed to monitor mRNA expression of p53-traget genes (i.e., Bax, p21, and MDM2). Data are expressed as fold change relative to Control cells (set to 1) and represent mean ± SEM of four independent experiments. Comparisons between Control and UBE3A − were performed using an unpaired two-tailed Student’s t -test; *** p < 0.001.

    Journal: International Journal of Molecular Sciences

    Article Title: Dysfunction of the Autophagy System and MDM2–p53 Axis Leads to the Accumulation of Amyloidogenic Proteins in Angelman Syndrome Models

    doi: 10.3390/ijms262211032

    Figure Lengend Snippet: p53 expression and activity in SH-SY5Y AS cellular model. ( A – D ) Cell lysates from SH-SY5Y (control and UBE3A − cells), were used for western blotting analysis using specific antibodies to total p53 ( A , B ) and phosphorylated p53 ( C , D ). A ‘stain-free protein normalization’ method was used for the normalization of bands to total protein in blots, eliminating the need for housekeeping proteins. Data are expressed as percentage versus control cells and are mean ± SEM of at least three independent experiments Comparisons between control and UB3A − were performed using an unpaired two-tailed Student’s t -test; * p < 0.05, ** p < 0.01, UBE3A − vs. control. ( E ) SHSY-5Y (control and UBE3A − ) cells were collected for RNA extraction and retro transcription to cDNA. A real-time PCR analysis was performed to monitor mRNA expression of p53-traget genes (i.e., Bax, p21, and MDM2). Data are expressed as fold change relative to Control cells (set to 1) and represent mean ± SEM of four independent experiments. Comparisons between Control and UBE3A − were performed using an unpaired two-tailed Student’s t -test; *** p < 0.001.

    Article Snippet: SH-SY5Y cells (control and UB3A − ) were collected, and total RNA was extracted using a RNeasyH Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions. cDNA synthesis was performed with 500 ng of RNA using the i-Script cDNA synthesis kit (BioRad, Hercules, CA, USA).

    Techniques: Expressing, Activity Assay, Control, Western Blot, Staining, Two Tailed Test, RNA Extraction, Real-time Polymerase Chain Reaction